Model of a repressilator based on three transcriptional repressors. a) Schematic representation of the repressilator circuit b) Simulation of optimal solution of parameter space search for target amplitude A = 100 and frequency f = 0.0001h−1. Parameters used: α0 = 2.288−7, α1 = 1.94, β = 0.0002, n = 4.199, γ = 0.0005, η = 0.007, k = 45.313, τ = 0.09. c) Comparison of parameters space investigation for amplitudes and frequencies of varying orders using Equations 9 and 10 (circles) or Equations 11 and 12 (squares). The y axis shows the relative amplitude error regarding the target amplitude. d) Comparison of parameters space investigation for amplitudes and frequencies of varying orders of magnitude Equations 9 and 10 (circles) or Equations 11 and 12 (squares). The y-axis shows the relative frequency error regarding the target frequency.